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Mapping transcription factors (TFs) occupancy is essential for understanding transcriptional programs. Here Akerberg, Gu, and colleagues use biotinylated knockin alleles of key cardiac TFs (GATA4, NKX2-5, MEF2A, MEF2C, SRF, TBX5, TEAD1) to map their genome-wide occupancy in the fetal and adult mouse heart, providing insight into the cardiac transcriptional regulatory network.